pdgf (R&D Systems)

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R&D Systems pdgf

( A ) MEF cells reconstituted with hPTP1B were grown on coverslips, serum starved, <t>and</t> <t>stimulated</t> with <t>PDGF</t> (50 ng/ml) for 4 min, and subsequently treated with 10 μM PDGFR inhibitor (PDGFRi) for 5, 10, and 20 min. Cells were fixed, permeabilized at the indicated times, and subsequently evaluated with primary antibodies from different species for hPTP1B (mouse) and GLRX1 (goat) to reveal protein-protein interactions in situ using PLA (red spots). Nuclei were labelled with 4′,6-diamidino-2-phenylindole (DAPI; blue). ( B ) Visualization and quantification were performed using fluorescent microscopy and ImageJ. Statistical differences in signal quantification are indicated in the bar graph (* P < 0.05; ** P < 0.01); n ≥ 3. Error bars denote SEM. ( C ) MEF cells reconstituted with hPTP1B were grown on coverslips, serum starved, and stimulated with PDGF (50 ng/ml) for 4 min or with 100 μM H 2 O 2 for 10 min, with and without catalase pretreatment (2 hours; 50 U/ml) as indicated. The images display results from one representative experiment, out of three independent experiments. Scale bars, 30 μm. Data were analyzed using one-way analysis of variance followed by Bonferroni post hoc tests for multiple comparisons with GraphPad Prism.

Pdgf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+pdgf+bb/Recombinant+Human+PDGF-BB+Protein%2C+CF/pmc13068064-242-11-12
Average 95 stars, based on 250 article reviews

pdgf - by Bioz Stars, 2026-10

95/100 stars

Images

1) Product Images from "Intracellular enzymatic reducing systems control receptor tyrosine kinase signaling via PTP1B"

Article Title: Intracellular enzymatic reducing systems control receptor tyrosine kinase signaling via PTP1B

Journal: Science Advances

doi: 10.1126/sciadv.adv5362

( A ) MEF cells reconstituted with hPTP1B were grown on coverslips, serum starved, and stimulated with PDGF (50 ng/ml) for 4 min, and subsequently treated with 10 μM PDGFR inhibitor (PDGFRi) for 5, 10, and 20 min. Cells were fixed, permeabilized at the indicated times, and subsequently evaluated with primary antibodies from different species for hPTP1B (mouse) and GLRX1 (goat) to reveal protein-protein interactions in situ using PLA (red spots). Nuclei were labelled with 4′,6-diamidino-2-phenylindole (DAPI; blue). ( B ) Visualization and quantification were performed using fluorescent microscopy and ImageJ. Statistical differences in signal quantification are indicated in the bar graph (* P < 0.05; ** P < 0.01); n ≥ 3. Error bars denote SEM. ( C ) MEF cells reconstituted with hPTP1B were grown on coverslips, serum starved, and stimulated with PDGF (50 ng/ml) for 4 min or with 100 μM H 2 O 2 for 10 min, with and without catalase pretreatment (2 hours; 50 U/ml) as indicated. The images display results from one representative experiment, out of three independent experiments. Scale bars, 30 μm. Data were analyzed using one-way analysis of variance followed by Bonferroni post hoc tests for multiple comparisons with GraphPad Prism.

Figure Legend Snippet: ( A ) MEF cells reconstituted with hPTP1B were grown on coverslips, serum starved, and stimulated with PDGF (50 ng/ml) for 4 min, and subsequently treated with 10 μM PDGFR inhibitor (PDGFRi) for 5, 10, and 20 min. Cells were fixed, permeabilized at the indicated times, and subsequently evaluated with primary antibodies from different species for hPTP1B (mouse) and GLRX1 (goat) to reveal protein-protein interactions in situ using PLA (red spots). Nuclei were labelled with 4′,6-diamidino-2-phenylindole (DAPI; blue). ( B ) Visualization and quantification were performed using fluorescent microscopy and ImageJ. Statistical differences in signal quantification are indicated in the bar graph (* P < 0.05; ** P < 0.01); n ≥ 3. Error bars denote SEM. ( C ) MEF cells reconstituted with hPTP1B were grown on coverslips, serum starved, and stimulated with PDGF (50 ng/ml) for 4 min or with 100 μM H 2 O 2 for 10 min, with and without catalase pretreatment (2 hours; 50 U/ml) as indicated. The images display results from one representative experiment, out of three independent experiments. Scale bars, 30 μm. Data were analyzed using one-way analysis of variance followed by Bonferroni post hoc tests for multiple comparisons with GraphPad Prism.

Techniques Used: Protein-Protein interactions, In Situ, Microscopy

Enzyme-linked Immunosorbent Assay:

Article Title: Characterization of Leukocyte- and Platelet-Rich Plasma Derived from Female Collage Athletes: A Cross-Sectional Cohort Study Focusing on Growth Factor, Inflammatory Cytokines, and Anti-Inflammatory Cytokine Levels
Article Snippet: .. As previously described [ ], the concentrations of PDGF-BB, platelet factor 4 (PF4), TGF-β1, IL-1β, and IL-1RA in frozen L-PRP were determined using human PDGF-BB, TGF-β1, PF4, IL-1β, and IL-1RA Quantikine ELISA kits (R&D Systems, Inc., Minneapolis, MN, USA). ..

Article Title: Characterization of platelet rich plasma in feline immunodeficiency virus-infected cats: Cell, and PDGF-BB and TGF-ß1 growth factor analysis.
Article Snippet: Autologous platelet-rich plasma (PRP) contains growth factors (GFs) that modulate the expression of inflammatory cells; thus, these products could be considered a good strategy to favor tissue regeneration in feline immunodeficiency (FIV) positive cats.. However, there is no scientific documentation on obtaining PRP in FIV-

Article Title: Characterization of Leukocyte- and Platelet-Rich Plasma Derived from Female Collage Athletes: A Cross-Sectional Cohort Study Focusing on Growth Factor, Inflammatory Cytokines, and Anti-Inflammatory Cytokine Levels.
Article Snippet: .. As previously described [16], the concentrations of PDGF-BB, platelet factor 4 (PF4), TGF-β1, IL-1β, and IL-1RA in frozen L-PRP were determined using human PDGF-BB, TGF-β1, PF4, IL-1β, and IL-1RA Quantikine ELISA kits (R&D Systems, Inc., Minneapolis, MN, USA). ..

Incubation:

Article Title: Metabolic alterations in human pulmonary artery smooth muscle cells treated with PDGF ‐ BB
Article Snippet: .. Human PASMCs were seeded at 1 × 10 4 cells per well with complete medium in 96‐well plates and incubated at 37°C with 5% CO 2 for 24 h. The cells were then serum starved in DMEM/F12 without FBS for another 24 h. Culture supernatants were discarded and replaced with DMEM/F12 with 20 ng/mL of human PDGF‐BB (220‐BB‐050, R&D Systems, MN, USA) or vehicle. ..

Article Title: Metabolic alterations in human pulmonary artery smooth muscle cells treated with PDGF-BB.
Article Snippet: .. Human PASMCs were seeded at 1 × 104 cells per well with complete medium in 96- well plates and incubated at 37°C with 5% CO2 for 24 h. The cells were then serum starved in DMEM/F12 without FBS for another 24 h. Culture supernatants were discarded and replaced with DMEM/F12 with 20 ng/mL of human PDGF- BB (220- BB- 050, R&D Systems, MN, USA) or vehicle. .. After incubation at 37°C with 5% CO2 for 24 or 48 h, cell viability and proliferation assays were performed as in our previous study.15 Briefly, to assess cell viability, culture supernatants were discarded and replaced with CCK- 8 solution (Dojindo Molecular Technologies Inc, Kumamoto, Japan), and the cells were incubated at 37°C with 5% CO2 for 1.5 h. Optical density (OD) was then determined at 450 nm using a microplate reader (Infinite M200 Pro, Tecan, Switzerland).

other:

Article Title: Manual platelet-rich plasma production in donkeys by double centrifugation results in leukocyte-rich platelet-rich plasma compared to single centrifugation.
Article Snippet: Platelet, WBC, and RBC enrichment factors of all products were calculated as the percentage ratio between the concentration of a parameter and its baseline value.21,23 The activities of PDGF-BB and TGF-β1 were analyzed in pools of extracted PRP and PPP, and baseline single-centrifugation and double-centrifugation plasma using a sandwich ELISA technique (human PDGF-BB and TGF-β1; Quantikine; R&D Systems) after 1 freeze-thaw cycle according to the manufacturer’s instructions as previously described.23 Validation was done to assess possible matrix effects within the assay when using donkeys by spiking 6 pool samples.

Concentration Assay:

Clinical Proteomics:

Isolation:

Article Title: SRF SUMOylation modulates smooth muscle phenotypic switch and vascular remodeling
Article Snippet: .. Aortic VSMCs isolated from WT and Senp1 SMCKO mice or MOVAS-1 cells were serum-starved for 24 h and stimulated with human PDGF-BB (10 ng/mL; Catalog #220-BB; R&D Systems, MN, USA) for different periods with or without AZD6244-pretreatment (0.5 μmol/L) for 12 h . Thereafter, VSMCs or MOVAS-1 cells were harvested for RNA and protein extraction or immunostaining. ..

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1) Product Images from "Intracellular enzymatic reducing systems control receptor tyrosine kinase signaling via PTP1B"

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Enzyme-linked Immunosorbent Assay:

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Clinical Proteomics:

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Immunostaining:

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